Email this article CYTOKINE PROFILE OF Th1-, Th2- AND Th17-LYMPHOCYTE SUBPOPULATIONS IN INFECTIOUS MYOCARDITIS
- Authors: Sanina N.P.1,2, Paleev F.N.1,2, Makarkov A.I.3,4, Mylov N.M.1,5, Ostrovskiy E.I.1,5, Khishova N.N.1,5, Moskalets O.V.1,5, Paleev N.R.1,5
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Affiliations:
- Moscow Regional Research and Clinical Institute (MONIKI)
- 61/2 Shchepkina ul., Moscow, 129110,Russian Federation
- Center of Family Planning and Reproduction of the Moscow City Health Department
- 3 Nezhinskaya ul., Moscow,119501, Russian Federation
- 61/2 Shchepkina ul., Moscow, 129110, Russian Federation
- Issue: No 35 (2014)
- Pages: 22-29
- Section: CARDIOLOGY
- URL: https://almclinmed.ru/jour/article/view/167
- DOI: https://doi.org/10.18786/2072-0505-2014-35-22-29
- ID: 167
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Abstract
Background: Type 17 T-helpers (Th17) were first identified more than 10 years ago. Though, there are only limited studies on the role of Th17- mediated mechanisms in the immune pathogenesis of acute myocarditis and dilative cardiomyopathy. Aim: To assess specific cytokine profile in patients with infectious immune myocarditis and post-myocarditis cardiosclerosis. Materials and methods: 35 patients with infectious immune myocarditis, 39 patients with post-myocarditis cardiosclerosis and 10 healthy volunteers (control group) were enrolled. 17 subjects with infectious immune myocarditis had manifest heart failure. Mean concentrations of Th1-, Th2- and Th17-derived cytokines were measured using solid-phase enzyme-linked immunosorbent assay panel manufactured by VectorBest ZAO (Russia). For statistical analysis, PASW Statistics software was used. Results: Compared to healthy controls, patients with infectious immune myocarditis had higher serum interferon-gamma (IF-γ) concentrations and more than 3-fold increase of serum interleukin (IL)-4. They also had almost 7-fold increase of IL-17A and Th17 effector cytokines – IL-8 and granulocyte-macrophage colony-stimulating factor (GM-CSF). Patients with infectious immune myocarditis and moderate or no symptoms of heart failure had significantly lower concentrations of IF-γ compared to patients with advanced heart failure. Cytokine concentrations peaked during the first 2 week of the disease, with following decrease. On the contrary, serum IL-4 grew significantly by the end of the first month and during the second month of the disease. Raised concentrations of IL-17A, IL-8 and GM-CSF were found throughout the period from the second week to the second month of the disease. Th17-derived cytokines concentrations were significantly increased in patients with post-myocarditis cardiosclerosis. Compared to the controls, IL-17A almost doubled, IL-8 increased by 51%, and GM-CSF – by 50%. Elevated concentrations of IL-4, IL-17A, IL-8 and GM-CSF were found in patients with duration of post-myocarditis cardiosclerosis less than 6 months. Conclusion: Disorders of anti-infective immunity and deficient self-limitation mechanisms of immune response play an important role in the development and progression of inflammatory myocardial diseases.
About the authors
N. P. Sanina
Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110,Russian Federation
Author for correspondence.
Email: natalyasanina2@yandex.ru
MD, PhD, Professor, Internal Diseases Department, Postgraduate Medical School Россия
F. N. Paleev
Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110,Russian Federation
Email: fake@neicon.ru
MD, PhD, Professor, Director Россия
A. I. Makarkov
Center of Family Planning and Reproduction of the Moscow City Health Department; 3 Nezhinskaya ul., Moscow,119501, Russian Federation
Email: fake@neicon.ru
PhD, Research Associate Россия
N. M. Mylov
Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation
Email: fake@neicon.ru
PhD, Associate Professor, Internal Diseases Department, Postgraduate Medical Schoo Россия
E. I. Ostrovskiy
Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation
Email: fake@neicon.ru
MD, PhD, Chief of the 1st Internal Diseases Department Россия
N. N. Khishova
Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation
Email: fake@neicon.ru
Fellow, Internal Diseases Department, Postgraduate Medical School Россия
O. V. Moskalets
Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation
Email: fake@neicon.ru
PhD, Chief Research Associate, Immunologic Laboratory Россия
N. R. Paleev
Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation
Email: fake@neicon.ru
Member of the Russian Academy of Sciences, MD, PhD, Professor, Chief of the Internal Diseases Department, Postgraduate Medical School Россия
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