Email this article Evaluation of an association of the rs1801133 MTHFR gene polymorphism with folic acid deficiency in obese patients
- Authors: Pogozheva A.V.1,2, Sorokina E.Y.1,2, Aristarkhova T.V.1
-
Affiliations:
- Federal Research Centre of Nutrition and Biotechnology
- I.M. Sechenov First Moscow State Medical University
- Issue: Vol 46, No 3 (2018)
- Pages: 254-257
- Section: ARTICLES
- URL: https://almclinmed.ru/jour/article/view/826
- DOI: https://doi.org/10.18786/2072-0505-2018-46-3-254-257
- ID: 826
Cite item
Full Text
Abstract
Background: The use of molecular genetic technologies has made it possible to show that the genetic factor plays a significant role in the development of obesity. In addition, in obese people the supply with vitamins, in particular with folic acid, is largely controlled genetically.
Aim: To study an association of the rs1801133 polymorphism of the MTHFR gene with folic acid deficiency in the residents of the Moscow region depending on their body mass index.
Materials and methods: rs1801133 polymorphisms were identified in 326 subjects (74 male and 252 female) aged from 20 to 65 years, living in the Moscow region. The DNA was isolated from blood by the sorption on silica gel-coated magnetic particles. DNA was isolated with the use of the epMotion 5075 automatic station (Eppendorf, Germany). To identify the polymorphism, a polymerase chain reaction was used, followed by cleavage of the Hinf1 restriction endonuclease products, with analysis of these products by gel electrophoresis. The equipment CFX96 Real Time System (BIO-RAD, USA) was used. Folic acid was measured by ID-Vit® Folic Acid test system (R-Biopharm, Germany).
Results: According to the results of folic acid measurements in blood, a deficiency of this vitamin was found in 24.2% of the studied residents of the Moscow region. Analysis of the genotyping results did not show any association of the rs1801133 MTHFR gene polymorphism with the serum levels of folic acid. However, in the subjects with overweight and obesity, there was a statistically significant association between the T allele of the rs1801133 of the MTHFR gene polymorphism and a low level of folic acid (odds ratio 2.5, 95% confidence interval 1.09–5.74, p = 0.03).
Conclusion: The rs1801133 polymorphism of the MTHFR gene significantly contributes to the development of folic acid deficiency in overweight and obese individuals.
About the authors
A. V. Pogozheva
Federal Research Centre of Nutrition and Biotechnology; I.M. Sechenov First Moscow State Medical University
Author for correspondence.
Email: allapogozheva@yandex.ru
Alla V. Pogozheva – MD, PhD, Leading Research Fellow, Laboratory of Epidemiology of Nutrition and Genodiagnostics of Alimentary-dependent Diseases FRCNB; Professor, Chair of Food Hygiene and Toxicology I.M. Sechenov FMSMU.
2/14 Ust'inskiy proezd, Moscow, 109240, Tel.: +7 (495) 698 53 80
РоссияE. Yu. Sorokina
Federal Research Centre of Nutrition and Biotechnology; I.M. Sechenov First Moscow State Medical University
Email: fake@neicon.ru
Elena Yu. Sorokina – MD, PhD, Leading Research Fellow, Laboratory of Epidemiology of Nutrition and Genodiagnostics of Alimentary-dependent Diseases FRCNB; Associate Professor, Chair of Food Hygiene and Toxicology FMSMU.
2/14 Ust'inskiy proezd, Moscow, 109240; 8/2 Trubetskaya ul., Moscow, 119991
РоссияT. V. Aristarkhova
Federal Research Centre of Nutrition and Biotechnology
Email: fake@neicon.ru
Tatyana V. Aristarkhova – Research Fellow, Laboratory of Metabolic and Proteomic Analysis.
2/14 Ust'inskiy proezd, Moscow, 109240
РоссияReferences
- Thomas-Valdés S, Tostes MDGV, Anunciação PC, da Silva BP, Sant'Ana HMP. Association between vitamin deficiency and metabolic disorders related to obesity. Crit Rev Food Sci Nutr. 2017;57(15): 3332–43. doi: 10.1080/10408398.2015.1117413.
- Liu X, Zhao LJ, Liu YJ, Xiong DH, Recker RR, Deng HW. The MTHFR gene polymorphism is associated with lean body mass but not fatbody mass. Hum Genet. 2008;123(2): 189–96. doi: 10.1007/s00439-007-0463-7.
- Tavakkoly Bazzaz J, Shojapoor M, Nazem H, Amiri P, Fakhrzadeh H, Heshmat R, Parvizi M, Hasani Ranjbar S, Amoli MM. Methylenetetrahydrofolate reductase gene polymorphism in diabetes and obesity. Mol Biol Rep. 2010;37(1): 105–9. doi: 10.1007/s11033-009-9545-z.
- Binia A, Contreras AV, Canizales-Quinteros S, Alonzo VA, Tejero ME, Silva-Zolezzi I. Geographical and ethnic distribution of single nucleotide polymorphisms within genes of the folate/homocysteine pathway metabolism. Genes Nutr. 2014;9(5): 421. doi: 10.1007/s12263-014-0421-7.
- Bailey LB, Stover PJ, McNulty H, Fenech MF, Gregory JF 3rd, Mills JL, Pfeiffer CM, Fazili Z, Zhang M, Ueland PM, Molloy AM, Caudill MA, Shane B, Berry RJ, Bailey RL, Hausman DB, Raghavan R, Raiten DJ. Biomarkers of Nutrition for Development – Folate Review. J Nutr. 2015;145(7): 1636S–80S. doi: 10.3945/jn.114.206599.
- Sun J, Xu Y, Xue J, Zhu Y, Lu H. Methylenetetrahydrofolate reductase polymorphism associated with susceptibility to coronary heart disease in Chinese type 2 diabetic patients. Mol Cell Endocrinol. 2005;229(1–2): 95–101. doi: 10.1016/j.mce.2004.09.003.
- Zhu B, Wu X, Zhi X, Liu L, Zheng Q, Sun G. Methylenetetrahydrofolate reductase C677T polymorphism and type 2 diabetes mellitus in Chinese population: a meta-analysis of 29 case-control studies. PLoS One. 2014;9(7):e102443. doi: 10.1371/journal.pone.0102443.
- Movva S, Alluri RV, Venkatasubramanian S, Vedicherla B, Vattam KK, Ahuja YR, Hasan Q. Association of methylene tetrahydrofolate reductase C677T genotype with type 2 diabetes mellitus patients with and without renal complications. Genet Test Mol Biomarkers. 2011;15(4): 257–61. doi: 10.1089/gtmb.2010.0118.
- Raza ST, Abbas S, Siddiqi Z, Mahdi F. Association between ACE (rs4646994), FABP2 (rs1799883), MTHFR (rs1801133), FTO (rs9939609) Genes Polymorphism and Type 2 Diabetes with Dyslipidemia. Int J Mol Cell Med. 2017;6(2): 121–30. doi: 10.22088/acadpub.BUMS.6.2.6.
- Karic A, Terzic R, Jerkic Z, Mustedanagic-Mujanovic J. The frequency of C677T methylenetetrahydrofolate reductase (MTHFR) polymorphism in Southern East Bosnian population. J Biomet Biostat. 2013;4(4): 1000169. doi: 10.4172/2155-6180.1000169.
Supplementary files
