Email this article EXPRESSION OF GENES RESPONSIBLE FOR MULTIDRUG RESISTANCE IN RELAPSED/REFRACTORY MULTIPLE MYELOMA PATIENTS
- Authors: Chernykh Y.B.1, Shushanov S.S.2
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Affiliations:
- Moscow Regional Research and Clinical Institute (MONIKI)
- N.N. Blokhin Russian Cancer Research Center of RAMS
- Issue: No 31 (2014)
- Pages: 47-51
- Section: ARTICLES
- URL: https://almclinmed.ru/jour/article/view/61
- DOI: https://doi.org/10.18786/2072-0505-2014-31-47-51
- ID: 61
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Abstract
Background: Multidrug resistance (MDR) is a natural phenomenon in development of solid and hematologic tumors. This phenomenon significantly influences both immediate and remote outcome of treatment. In this connection, an interest arises to studying this problem in multiple myeloma (MM) patients with expressed clinical signs of tumorous progression, the main of them being an absence or loss of the response to antitumor treatment. Aim: To study the expression of messenger RNA (mRNA) of a series of MDR genes, namely MDR1, MRP1, LRP, BCRP responsible for MDR development in the bone marrow aspirate of resistant patients prior to bortezomib-containing chemotherapy. Materials and methods: Study group included 19 relapsed/refractory multiple myeloma patients. Investigation of MDR genes expression was carried out on the cells of the bone marrow mononuclear fraction containing plasmocytes. The mRNA level was analyzed by semiquantitative RT-PCR (polymerase chain reaction with reverse transcription). Patients were treated with bortezomib-containing chemotherapy hereafter. Results: mRNA expression of genes MDR1, MRP1, BCRP was revealed in all (100%) patients, and that of gene LRP – in 81% of myeloma samples. The levels of MDR genes mRNA expression were different. On this basis, two groups of patients were identified: with the levels of MDR genes expression above and beyond the average. Conclusion: 100%-expression of MDR genes (MDR1, MRP1, BCRP) mRNA was revealed in drug resistant MM patients. The median survival in group of patients with higher levels of MDR genes mRNA expression versus lower levels of MDR genes mRNA expression was statistically significant.
About the authors
Yu. B. Chernykh
Moscow Regional Research and Clinical Institute (MONIKI)
Author for correspondence.
Email: yulia_chernih@mail.ru
scientific worker, Department of Clinical Hematology and Immunotherapy of MONIKI Россия
S. S. Shushanov
N.N. Blokhin Russian Cancer Research Center of RAMS
Email: fake@neicon.ru
PhD, senior scientific worker, Laboratory of tumor cell genetics, Blokhin RCRC Россия
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