ASSOCIATION OF SEBORRHEIC KERATOSIS AND HUMAN PAPILLOMA VIRUS IN IMMUNE-SUPPRESSED AND IMMUNOCOMPETENT PATIENTS: A COMPARISON STUDY

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Abstract

Aim: To study an association between seborrheic keratosis and human papilloma virus (HPV) using quantitative analysis of viral desoxyribonucleic acid (DNA); to assess prevalence of different phenotypes of beta-HPV. Materials and methods: We examined 60 renal transplant recipients (20 of them had multiple seborrheic keratosis) and 22 immunocompetent patients with seborrheic keratosis. Control group included 49 healthy subjects. Burr biopsy samples (micro-samples) were collected in sterile conditions. After sample procession and DNA isolation using DNK-sorb-C kit (Central Research Institute for Epidemiology – CRIE), polymerase chain reaction for HPV was performed with real-time fluorescent hybridization detection. For DNA amplification and detection we used RotorGene 3000 analyzer (Corbett Research, Australia). In the beta-HPV assay, recombinant plasmids were used as positive controls and control human beta-globin gene fragments (CRIE). 4 oligo-nucleotide systems (group-specific primers and probes) were used for the detection of beta-HPV DNA. Results: Keratotic lesions of open and covered skin regions were common in renal transplant recipients. Beta-HPV DNA was more frequent in seborrheis keratomas and intact skin (81% and 55%) of renal transplant recipients compared to healthy donors (47%). Conclusion: HPV DNA was frequently detected in keratotic lesions and intact skin of renal transplant recipients. In immunocompetent patients prevalence of HPV DNA in keratotic lesions was significantly higher compared to intact skin.

About the authors

V. A. Molochkov

Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation

Email: fake@neicon.ru
MD, PhD, Professor, Head of the Dermatovenereology and Dermato-Oncology Department Russian Federation

L. V. Korneva

Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation

Author for correspondence.
Email: lvkorneva@mail.ru
MD, PhD, Assistant Professor, Dermatovenereology and Dermato-Oncology Department Russian Federation

E. S. Snarskaya

I.M. Sechenov First Moscow State Medical University; 8/2 Trubetskaya ul., Moscow, 119991, Russian Federation

Email: fake@neicon.ru
MD, PhD, Professor, Department of Skin and Venereal Diseases, Institute for Professional Education Russian Federation

E. O. Shcherbakova

Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation

Email: fake@neicon.ru
MD, PhD, Senior Research Associate, Chronic Dialysis and Kidney Transplantation Department Russian Federation

A. A. Polyanskaya

Medical Aesthetics Clinic “Premium Aesthetics”; 3–6 Kazarmennyy pereulok, Moscow, 109028, Russian Federation

Email: fake@neicon.ru
Dermatovenereologist, Cosmetologist Russian Federation

O. B. Trofimova

Central Research Institute for Epidemiology; 3а Novogireevskaya ul., Moscow, 111123, Russian Federation

Email: fake@neicon.ru
Junior Research Associate, Molecular Diagnosis and Epidemiology Department Russian Federation

References

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  2. Куевда ДА, Кладова АЮ, Шипулина ОЮ, Молочков ВА. Разработка методики количественного определения папилломавирусов рода бета. В: Материалы VI научно-практической конференции «Социально значимые заболевания в дерматовенерологии. Диагностика, терапия, профилактика». М.; 2006. с. 97–8. (Kuevda DA, Kladova AYu, Shipulina OYu, Mo-lochkov VA. Development of beta-pa pilloma virus assay. In: Proceedings of the VI Research-to-Practice Conference “Socially Significant Diseases in Dermatovenereology. Diagnosis, Therapy, Prevention”. Moscow; 2006. p. 97–8. Russian).
  3. Галил-Оглы ГА, Молочков ВА, Сергеев ЮВ. Дерматоонкология. М.: Медицина; 2005. 865 с. (Galil-Ogly GA, Molochkov VA, Sergeev YuV. Dermato-Oncology. Moscow: Meditsina; 2005. 865 р. Russian).
  4. Asgari MM, Kiviat NB, Critchlow CW, Stern JE, Argenyi ZB, Raugi GJ, Berg D, Odland PB, Hawes SE, de Villiers EM. Detection of human papillomavirus DNA in cutaneous squamous cell carcinoma among immunocompetent individuals. J Invest Dermatol. 2008;128(6): 1409–17.

Copyright (c) 2016 Molochkov V.A., Korneva L.V., Snarskaya E.S., Shcherbakova E.O., Polyanskaya A.A., Trofimova O.B.

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