DETERMINATION OF FUNDAMENTAL PHYSICAL PARAMETERS OF BLOOD SERUM PROTEINS FOR DEVELOPMENT THE METHODS FOR CANCER DIAGNOSIS

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Abstract

Background: A fundamental problem of medical diagnostics as an assessment of human plasma and serum proteins in various pathologic states. Physical molecular and dynamic characteristics of proteins that are significantly changed during a  disease have been proposed as diagnostic parameters. Aim: To conduct fundamental research on molecular properties of serum proteins, such as their mobility, intermolecular interactions, aggregation/formation of nanoscale protein clusters, abnormalities of the surface charge, etc., for subsequent development of methods for early diagnostics of cancer based on laser light scattering and fluorescence. Materials and methods: The parameters of protein dynamics were assessed by laser light scattering methods. Results: We performed comparative assessments in model systems and native serum samples for cancer diagnostics and analyzed the possibility to use optical parameters of water serum solutions for development of cancer diagnostic methods. Conclusion: Light scattering methods provide sufficient information on changes of the static and dynamic properties of the serum proteins over time during various pathological conditions.

About the authors

V. V. Gibizova

Lomonosov Moscow State University; 1 Leninskie gory, Moscow, 119991, Russian Federation

Email: fedorova@physics.msu.ru
PhD Student, Chair of Molecular Processes and Extreme Matter States, Faculty of Physics Russian Federation

K. A. Anenkova

Lomonosov Moscow State University; 1 Leninskie gory, Moscow, 119991, Russian Federation

Email: fedorova@physics.msu.ru
PhD Student, Chair of Molecular Processes and Extreme Matter States, Faculty of Physics Russian Federation

A. D. Maslennikova

Lomonosov Moscow State University; 1 Leninskie gory, Moscow, 119991, Russian Federation

Email: fedorova@physics.msu.ru
Specialist on Education and Methodology, Department of Education, Deanery of the Faculty of Physics Russian Federation

K. V. Fedorova

Lomonosov Moscow State University; 1 Leninskie gory, Moscow, 119991, Russian Federation

Author for correspondence.
Email: fedorova@physics.msu.ru
Assistant, Chair of Molecular Processes and Extreme Matter States, Faculty of Physics Russian Federation

I. A. Sergeeva

Lomonosov Moscow State University; 1 Leninskie gory, Moscow, 119991, Russian Federation

Email: fedorova@physics.msu.ru
PhD (in Physics and Mathematics), Senior Lecturer, Chair of Molecular Processes and Extreme Matter States, Faculty of Physics Russian Federation

G. P. Petrova

Lomonosov Moscow State University; 1 Leninskie gory, Moscow, 119991, Russian Federation

Email: fedorova@physics.msu.ru
PhD (in Physics and Mathematics), Professor, Chair of Molecular Processes and Extreme Matter States, Faculty of Physics Russian Federation

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Copyright (c) 2016 Gibizova V.V., Anenkova K.A., Maslennikova A.D., Fedorova K.V., Sergeeva I.A., Petrova G.P.

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