Association of rs266729 and rs16861194 polymorphisms of the ADIPOQ gene with the risk of obesity in residents of the Moscow region

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Abstract

Rationale: The contribution of the adiponectin gene polymorphisms (ADIPOQ, located at 3q27) in the residents of Nigeria and China to the risk of overweight and its association with the risk of arterial hypertension in the European population have been demonstrated.

Aim: To identify associations between rs266729 and rs16861194 polymorphisms of the ADIPOQ gene with overweight and obesity in the Moscow region residents.

Materials and methods: Identification of rs266729 and rs16861194 polymorphisms of the ADIPOQ gene was carried out in 222 people (140 women and 82 men, aged 25 to 65 years) living in the Moscow region. Genotyping was performed using allele-specific amplification with real-time detection of the results on the CFX96 Real-Time System amplifier (Bio-Rad, USA) and using TaqMan probes complementary to polymorphic DNA regions. To assess an association between these genetic polymorphisms and overweight/obesity, we performed a case control study, with the cases being subjects with body mass index (BMI) of ≥ 30 kg/m2, and the controls those with BMI of < 30 kg/m2.

Results: The mean frequency of the minor allele G rs266729 polymorphism of the ADIPOQ gene in the subjects from the Moscow region was 26.8% and similar in men and women. Comparison of the CC and GG genotypes carriers of the rs266729 polymorphism of the ADIPOQ gene in men showed a statistically significant association of the GG genotype to the BMI value (p = 0.04). There were no statistically significant differences between anthropometric indicators (BMI, body fat mass) in the carriers of different rs16861194 polymorphism genotypes of the ADIPOQ. No association between the studied polymorphisms and blood glucose levels and lipid spectrum could be found.

Conclusion: The frequency of the minor allele G of the rs266729 polymorphism of the ADIPOQ gene in the subjects from the Moscow region was similar to their rates in the Russian Federation and European countries. In Moscow residents, the rs266729 polymorphism of the ADIPOQ gene (G allele) contributes to the risk of obesity in homozygous carriers (genotype GG). No association of the rs16861194 polymorphism of the ADIPOQ gene with the BMI was found; therefore, this polymorphism cannot be considered as a genetic marker of the obesity risk.

About the authors

A. V. Pogozheva

Federal Research Centre of Nutrition, Biotechnology and Food Safety;
I.M. Sechenov First Moscow State Medical University

Author for correspondence.
Email: allapogozheva@yandex.ru
ORCID iD: 0000-0003-4619-291X

Alla V. Pogozheva – MD, PhD, Professor, Leading Research Fellow, Laboratory of Demography and Epidemiology of Nutrition Federal Research Centre of Nutrition, Biotechnology and Food Safety; Professor, Chair of Food Hygiene and Toxicology I.M. Sechenov First Moscow State Medical University

2/14 Ust'inskiy proezd, Moscow, 109240; 

8/2 Trubetskaya ul., Moscow, 119991

Russian Federation

E. Yu. Sorokina

Federal Research Centre of Nutrition, Biotechnology and Food Safety;
I.M. Sechenov First Moscow State Medical University

Email: sorokina@ion.ru
ORCID iD: 0000-0002-6530-6233

Elena Yu. Sorokina – MD, PhD, Leading Research Fellow, Laboratory of Demography and Epidemiology of Nutrition Federal Research Centre of Nutrition, Biotechnology and Food Safety; Associate Professor, Chair of Food Hygiene and Toxicology I.M. Sechenov First Moscow State Medical University

2/14 Ust'inskiy proezd, Moscow, 109240;

8/2 Trubetskaya ul., Moscow, 119991

Russian Federation

References

  1. Терещенко ИВ, Каменских ЯА, Суслина АА. Адипонектин в норме и патологии. Терапевтический архив. 2016;88(12): 126–132. doi: 10.17116/terarkh20168812126-132.
  2. Larifla L, Rambhojan C, Joannes MO, Maimaitiming-Madani S, Donnet JP, Marianne-Pépin T, Chout R, Roussel R, Foucan L. Gene polymorphisms of FABP2, ADIPOQ and ANP and risk of hypertriglyceridemia and metabolic syndrome in Afro-Caribbeans. PLoS One. 2016;11(9):e0163421. doi: 10.1371/journal.pone.0163421.
  3. Петренко ЮВ, Герасимова КС, Новикова ВП. Биологическая и патофизиологическая значимость адипонектина. Педиатрия. 2019;10(2): 83–87. doi: 10.17816/PED10283-87.
  4. Cao Y, Tao L, Yuan Y, Jiao X, Lau WB, Wang Y, Christopher T, Lopez B, Chan L, Goldstein B, Ma XL. Endothelial dysfunction in adiponectin deficiency and its mechanisms involved. J Mol Cell Cardiol. 2009;46(3): 413–419. doi: 10.1016/j.yjmcc.2008.10.014.
  5. Peralta Romero JJ, Karam Araujo R, Burguete García AI, Estrada Velasco BI, López Islas C, Figueroa Arredondo PMDC, Valladares Salgado A, Cruz M. ADIPOQ and ADIPOR2 gene polymorphisms: association with overweight/ obesity in Mexican children. Bol Med Hosp Infant Mex. 2015;72(1): 26–33. doi: 10.1016/j.bmhimx.2015.02.002.
  6. He J, Stryjecki C, Reddon H, Peralta-Romero J, Karam-Araujo R, Suarez F, Gomez-Zamudio J, Burguete-Garcia A, Alyass A, Cruz M, Meyre D. Adiponectin is associated with cardio-metabolic traits in Mexican children. Sci Rep. 2019;9(1): 3084. doi: 10.1038/s41598-019-39801-2.
  7. Hwang JY, Park JE, Choi YJ, Huh KB, Chang N, Kim WY. Carbohydrate intake interacts with SNP276G>T polymorphism in the adiponectin gene to affect fasting blood glucose, HbA1C, and HDL cholesterol in Korean patients with type 2 diabetes. J Am Coll Nutr. 2013;32(3): 143–150. doi: 10.1080/07315724.2013.791795.
  8. Gajewska J, Kuryłowicz A, Ambroszkiewicz J, Mierzejewska E, Chełchowska M, Szamotulska K, Weker H, Puzianowska-Kuźnicka M. ADIPOQ -11377C>G Polymorphism increases the risk of adipokine abnormalities and child obesity regardless of dietary intake. J Pediatr Gastroenterol Nutr. 2016;62(1): 122–129. doi: 10.1097/MPG.0000000000000900.
  9. Warodomwichit D, Shen J, Arnett DK, Tsai MY, Kabagambe EK, Peacock JM, Hixson JE, Straka RJ, Province MA, An P, Lai CQ, Parnell LD, Borecki IB, Ordovas JM. ADIPOQ polymorphisms, monounsaturated fatty acids, and obesity risk: the GOLDN study. Obesity (Silver Spring). 2009;17(3): 510–517. doi: 10.1038/oby.2008.583.
  10. Бровин ДЛ, Драчева КВ, Пантелеева АА, Беляева ОД, Пчелина СН, Баженова ЕА, Каронова ТЛ, Колодина ДА, Полякова ЕА, Волкова АР, Козлова СН, Беркович ОА, Баранова ЕИ. Варианты гена адипонектина (ADIPOQ) rs2441766 и rs266729: ассоциация с концентрацией общего и высокомолекулярного адипонектина сыворотки крови у женщин с абдоминальным ожирением и метаболическим синдромом. Медицинская генетика. 2019;18(1): 25–34. doi: 10.25557/2073-7998.2019.01.25-34.
  11. Divella R, Daniele A, Mazzocca A, Abbate I, Casamassima P, Caliandro C, Ruggeri E, Naglieri E, Sabbà C, De Luca R. ADIPOQ rs266729 G/C gene polymorphism and plasmatic adipocytokines connect metabolic syndrome to colorectal cancer. J Cancer. 2017;8(6): 1000–1008. doi: 10.7150/jca.17515.
  12. Nascimento H, Vieira E, Coimbra S, Catarino C, Costa E, Bronze-da-Rocha E, Rocha-Pereira P, Carvalho M, Ferreira Mansilha H, Rêgo C, Dos Santos R, Santos-Silva A, Belo L. Adipokine gene single-nucleotide polymorphisms in Portuguese obese adolescents: Associations with plasma concentrations of adiponectin, resistin, IL-6, IL-1β, and TNF-α. Child Obes. 2016;12(4): 300–313. doi: 10.1089/chi.2015.0235.
  13. Fan W, Qu X, Li J, Wang X, Bai Y, Cao Q, Ma L, Zhou X, Zhu W, Liu W, Ma Q. Associations between polymorphisms of the ADIPOQ gene and hypertension risk: a systematic and meta-analysis. Sci Rep. 2017;7:41683. doi: 10.1038/srep41683.
  14. Ogundele OE, Adekoya KO, Osinubi AA, Awofala AA, Oboh BO. Association of adiponectin gene (ADIPOQ) polymorphisms with measures of obesity in Nigerian young adults. Egyptian Journal of Medical Human Genetics. 2018;19(2): 123–127. doi: 10.1016/j.ejm-hg.2017.08.005.
  15. Wu J, Liu Z, Meng K, Zhang L. Association of adiponectin gene (ADIPOQ) rs2241766 polymorphism with obesity in adults: a meta-analysis. PLoS One. 2014;9(4):e95270. doi: 10.1371/journal.pone.0095270.
  16. Zhou JM, Zhang M, Wang S, Wang BY, Han CY, Ren YC, Zhang L, Zhang HY, Yang XY, Zhao Y, Hu DS. Association of the ADIPOQ Rs2241766 and Rs266729 polymorphisms with metabolic syndrome in the Chinese population: A Meta-analysis. Biomed Environ Sci. 2016;29(7): 505–515. doi: 10.3967/bes2016.066.
  17. Chu H, Wang M, Zhong D, Shi D, Ma L, Tong N, Zhang Z. AdipoQ polymorphisms are associated with type 2 diabetes mellitus: a meta-analysis study. Diabetes Metab Res Rev. 2013;29(7): 532–545. doi: 10.1002/dmrr.2424.
  18. Patel S, Flyvbjerg A, Kozàkovà M, Frystyk J, Ibrahim IM, Petrie JR, Avery PJ, Ferrannini E, Walker M; RISC Investigators. Variation in the ADIPOQ gene promoter is associated with carotid intima media thickness independent of plasma adiponectin levels in healthy subjects. Eur Heart J. 2008;29(3): 386–393. doi: 10.1093/eurheartj/ehm526.
  19. Shi H, Lu Y, Du J, Du W, Ye X, Yu X, Ma J, Cheng J, Gao Y, Cao Y, Zhou L, Li Q. Application of back propagation artificial neural network on genetic variants in adiponectin ADIPOQ, peroxisome proliferator-activated receptor-γ, and retinoid X receptor-α genes and type 2 diabetes risk in a Chinese Han population. Diabetes Technol Ther. 2012;14(3): 293–300. doi: 10.1089/dia.2011.0071.
  20. Siitonen N, Pulkkinen L, Lindström J, Kolehmainen M, Eriksson JG, Venojärvi M, Ilanne-Parikka P, Keinänen-Kiukaanniemi S, Tuomilehto J, Uusitupa M. Association of ADIPOQ gene variants with body weight, type 2 diabetes and serum adiponectin concentrations: the Finnish Diabetes Prevention Study. BMC Med Genet. 2011;12:5. doi: 10.1186/1471-2350-12-5.
  21. Zemlin AE, Matsha TE, Kengne AP, Hon G, Erasmus RT. High Molecular Weight Adiponectin Levels are neither influenced by adiponectin polymorphisms nor associated with insulin resistance in mixed-ancestry hyperglycemic subjects from South Africa. J Med Biochem. 2016;35(4): 416–427. doi: 10.1515/jomb-2016-0024.
  22. Tanimura D, Shibata R, Izawa H, Hirashiki A, Asano H, Murase Y, Miyata S, Nakatochi M, Ouchi N, Ichihara S, Yasui K, Yoshida T, Naruse K, Matsubara T, Yokota M. Relation of a common variant of the adiponectin gene to serum adiponectin concentration and metabolic traits in an aged Japanese population. Eur J Hum Genet. 2011;19(3): 262–269. doi: 10.1038/ejhg.2010.201.
  23. Jiang B, Liu Y, Liu Y, Fang F, Wang X, Li B. Association of four insulin resistance genes with type 2 diabetes mellitus and hypertension in the Chinese Han population. Mol Biol Rep. 2014;41(2): 925–933. doi: 10.1007/s11033-013-2937-0.
  24. Погожева АВ, Сорокина ЕЮ, Сокольников АА. Ассоциации ожирения с обеспеченностью витамином D в зависимости от полиморфизмов rs2228570 гена VDR и rs9939609 гена FTO у жителей средней полосы и Крайнего Севера России. Альманах клинической медицины. 2019;47(2): 112–119. doi: 10.18786/2072-0505-2019-47-015.

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Copyright (c) 2021 Pogozheva A.V., Sorokina E.Y.

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