THE INFLUENCE OF NEUTRALIZING ANTIBODIES TO INTERFERON-BETA ON PROGRESSION OF MULTIPLE SCLEROSIS

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Abstract

Background: Neutralizing antibodies (NAbs) affect the effectiveness of interferon therapy in patients with multiple sclerosis; however, this influence cannot be considered as unequivocal. NAbs formation is determined by several factors, such as frequency and duration of administration, interferon-β (IFN-β) formulation and the patient's genotype. It has been found that NAb titers vary over time. Aim: To assess the levels of NAbs to IFN-β in patients with multiple sclerosis and to investigate their relationship to disease progression. Materials and methods: We analyzed serum samples from 83  multiple sclerosis patients after a  long-term IFN-β-1b treatment; NAbs detection reaction was performed by assessment of their cytopathic effect. Results: NAbs were found in 63.9%  (53  of 83) of patients with dura￾tion of IFN-β-1b treatment of 33.3±17.6 months. All NAb-positive patients were treated with high￾dose IFN-β. Patients with titers>800  LU (n=28) demonstrated a  trend towards more advanced neurologic deficit on the Expanded Disability Status Scale (EDSS), compared to the patients with normal NAbs titers (0  to 20  LU, n=30) and intermediate titers (20 to 800 LU, n=25) (p>0.05). The exacerbation rate in the group with NAbs titers from 20  to 800  LU was insignificantly lower than that in the group with NAbs>800 and in the NAb-negative patients (p>0.05). Conclusion: Testing for NAbs may be a promising method for monitoring of IFN-β therapy in multiple sclerosis. There was a trend towards more pronounced neurological deficit in patients with high NAbs titers, but paradoxical data on a high rate of exacerbations in NAb-negative patients requires further study. 

About the authors

V. Yu. Lizhdvoy

Moscow Regional Research and Clinical Institute (MONIKI)

Author for correspondence.
Email: lijdvoy@mail.ru

MD, Senior Research Fellow, Department of Neurology

61/2–10 Shchepkina ul., Moscow, 129110

Tel.: +7 (495) 684 57 38

Россия

T. P. Ospel'nikova

N.F. Gamaleya Research Institute of Epidemiology and Microbiology

Email: fake@neicon.ru

MD, Senior Research Fellow, Laboratory of Interferonogenesis

18 Gamalei ul., Moscow, 123098

Россия

S. V. Kotov

Moscow Regional Research and Clinical Institute (MONIKI)

Email: fake@neicon.ru

MD, PhD, Professor; Head of Department of Neurology; Head of Chair of Neurology, Postgraduate Training Faculty

61/2 Shchepkina ul., Moscow, 129110

Россия

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Copyright (c) 2016 Lizhdvoy V.Y., Ospel'nikova T.P., Kotov S.V.

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