Investigation of the formation of cardiac tissue on substrates of varying degrees of anisotropy and rigidity

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Abstract

In the last decade, in vitro experiments have shown that mechanical properties of the bases could markedly influence the efficacy of differentiation of the induced pluripotent and embryonic stem cells and their development into the mature phenotype. By changing of mechanical, elastic and structural characteristics of the base, it is possible to increase the percentage of stem cells that differentiate to cardiomyocytes. The study was aimed at evaluation of the effects induced by changing physical characteristics of the base on the formation of phenotypic characteristics of cardiac cells. This included the comparison of structural properties of the cultured layer of heart tissue obtained by changing of elasticity and structure of polymeric bases. The results showed significant differences in calcium activity and structural characteristics of cardiomyocytes depending on the base properties, as well as significant variation in the excitation conduction. As long as conduction abnormalities in the heart tissues can often lead to occurrence of life-threatening cardiac arrhythmias, the results can be used to determine patient groups at increased risk of death from heart failure.

About the authors

S. A. Shcherbina

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0002-4223-0133

Serafima A. Shcherbina – Master's Student, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Россия

A. V. Shutko

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0002-1921-571X

Angelina V. Shutko – Master, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Россия

A. A. Nizamieva

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0002-7162-5214

Aygul A. Nizamieva – Postgraduate Student, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Россия

A. V. Nikitina

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0002-6462-7027

Anna V. Nikitina – Master, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Россия

M. M. Slotvitsky

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0003-2237-4217

Mikhail M. Slotvitsky – Postgraduate Student, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Россия

V. A. Tsvelaya

Moscow Institute of Physics and Technology;
Moscow Regional Research and Clinical Institute (MONIKI)

Author for correspondence.
Email: v.tsvelaya@monikiweb.ru
ORCID iD: 0000-0002-3554-9736

Valeria A. Tsvelaya – PhD (in Biol.), Research Fellow, Laboratory of Biophysics of Excitable Systems Moscow Institute of Physics and Technology; Senior Research Fellow, Laboratory of Molecular and Cell Diagnostics Moscow Regional Research and Clinical Institute (MONIKI)

9 Institutskiy per., Dolgoprudny, 140180;

61/2–8 Shchepkina ul., Moscow, 129110

Россия

K. I. Agladze

Moscow Institute of Physics and Technology; Moscow Regional Research and Clinical Institute (MONIKI)

Email: fake@neicon.ru
ORCID iD: 0000-0002-9258-436X

Konstantin I. Agladze – Doctor of Biol. Sci., Professor, Head of Laboratory of Biophysics of Excitable Systems Moscow Institute of Physics and Technology; Head of Laboratory of Molecular and Cell Diagnostics Moscow Regional Research and Clinical Institute (MONIKI)

9 Institutskiy per., Dolgoprudny, 140180;

61/2 Shchepkina ul., Moscow, 129110

Россия

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Copyright (c) 2021 Shcherbina S.A., Shutko A.V., Nizamieva A.A., Nikitina A.V., Slotvitsky M.M., Tsvelaya V.A., Agladze K.I.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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