Investigation of the formation of cardiac tissue on substrates of varying degrees of anisotropy and rigidity

Cover Page


Cite item

Full Text

Abstract

In the last decade, in vitro experiments have shown that mechanical properties of the bases could markedly influence the efficacy of differentiation of the induced pluripotent and embryonic stem cells and their development into the mature phenotype. By changing of mechanical, elastic and structural characteristics of the base, it is possible to increase the percentage of stem cells that differentiate to cardiomyocytes. The study was aimed at evaluation of the effects induced by changing physical characteristics of the base on the formation of phenotypic characteristics of cardiac cells. This included the comparison of structural properties of the cultured layer of heart tissue obtained by changing of elasticity and structure of polymeric bases. The results showed significant differences in calcium activity and structural characteristics of cardiomyocytes depending on the base properties, as well as significant variation in the excitation conduction. As long as conduction abnormalities in the heart tissues can often lead to occurrence of life-threatening cardiac arrhythmias, the results can be used to determine patient groups at increased risk of death from heart failure.

About the authors

S. A. Shcherbina

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0002-4223-0133

Serafima A. Shcherbina – Master's Student, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Russian Federation

A. V. Shutko

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0002-1921-571X

Angelina V. Shutko – Master, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Russian Federation

A. A. Nizamieva

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0002-7162-5214

Aygul A. Nizamieva – Postgraduate Student, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Russian Federation

A. V. Nikitina

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0002-6462-7027

Anna V. Nikitina – Master, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Russian Federation

M. M. Slotvitsky

Moscow Institute of Physics and Technology

Email: fake@neicon.ru
ORCID iD: 0000-0003-2237-4217

Mikhail M. Slotvitsky – Postgraduate Student, Engineer, Laboratory of Biophysics of Excitable Systems

9 Institutskiy per., Dolgoprudny, 140180

Russian Federation

V. A. Tsvelaya

Moscow Institute of Physics and Technology;
Moscow Regional Research and Clinical Institute (MONIKI)

Author for correspondence.
Email: v.tsvelaya@monikiweb.ru
ORCID iD: 0000-0002-3554-9736

Valeria A. Tsvelaya – PhD (in Biol.), Research Fellow, Laboratory of Biophysics of Excitable Systems Moscow Institute of Physics and Technology; Senior Research Fellow, Laboratory of Molecular and Cell Diagnostics Moscow Regional Research and Clinical Institute (MONIKI)

9 Institutskiy per., Dolgoprudny, 140180;

61/2–8 Shchepkina ul., Moscow, 129110

Russian Federation

K. I. Agladze

Moscow Institute of Physics and Technology; Moscow Regional Research and Clinical Institute (MONIKI)

Email: fake@neicon.ru
ORCID iD: 0000-0002-9258-436X

Konstantin I. Agladze – Doctor of Biol. Sci., Professor, Head of Laboratory of Biophysics of Excitable Systems Moscow Institute of Physics and Technology; Head of Laboratory of Molecular and Cell Diagnostics Moscow Regional Research and Clinical Institute (MONIKI)

9 Institutskiy per., Dolgoprudny, 140180;

61/2 Shchepkina ul., Moscow, 129110

Russian Federation

References

  1. Besser RR, Ishahak M, Mayo V, Carbonero D, Claure I, Agarwal A. Engineered Microenvironments for Maturation of Stem Cell Derived Cardiac Myocytes. Theranostics. 2018;8(1): 124–140. doi: 10.7150/thno.19441.
  2. Mummery CL, Zhang J, Ng ES, Elliott DA, Elefanty AG, Kamp TJ. Differentiation of human embryonic stem cells and induced pluripotent stem cells to cardiomyocytes: a methods overview. Circ Res. 2012;111(3): 344–358. doi: 10.1161/CIRCRESAHA.110.227512.
  3. Lundy SD, Zhu WZ, Regnier M, Laflamme MA. Structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells. Stem Cells Dev. 2013;22(14): 1991– 2002. doi: 10.1089/scd.2012.0490.
  4. Slotvitsky M, Tsvelaya V, Frolova S, Dementyeva E, Agladze K. Arrhythmogenicity Test Based on a Human-Induced Pluripotent Stem Cell (iP-SC)-Derived Cardiomyocyte Layer. Toxicol Sci. 2019;168(1): 70–77. doi: 10.1093/toxsci/kfy274.
  5. Slotvitsky MM, Tsvelaya VA, Podgurskaya AD, Agladze KI. Formation of an electrical coupling between differentiating cardiomyocytes. Sci Rep. 2020;10(1): 7774. doi: 10.1038/s41598-020-64581-5.
  6. Herron TJ, Rocha AM, Campbell KF, Ponce-Balbuena D, Willis BC, Guerrero-Serna G, Liu Q, Klos M, Musa H, Zarzoso M, Bizy A, Furness J, Anumonwo J, Mironov S, Jalife J. Extracellular Matrix-Mediated Maturation of Human Pluripotent Stem Cell-Derived Cardiac Monolayer Structure and Electrophysiological Function. Circ Arrhythm Electrophysiol. 2016;9(4):e003638. doi: 10.1161/CIRCEP.113.003638.
  7. LaBarge W, Mattappally S, Kannappan R, Fast VG, Pretorius D, Berry JL, Zhang J. Maturation of three-dimensional, hiPSC-derived cardiomyocyte spheroids utilizing cyclic, uniaxial stretch and electrical stimulation. PLoS One. 2019;14(7):e0219442. doi: 10.1371/journal.pone.0219442.
  8. Слотвицкий ММ, Цвелая ВА, Фролова ШР, Дементьева ЕВ, Агладзе КИ. Исследование функциональности получаемых из индуцированных плюрипотентных стволовых клеток кардиомиоцитов для моделирования сердечных аритмий при синдроме удлиненного интервала QT. Вавиловский журнал генетики и селекции. 2018;22(2): 187–195. doi: 10.18699/VJ18.346.
  9. Lian X, Zhang J, Azarin SM, Zhu K, Hazeltine LB, Bao X, Hsiao C, Kamp TJ, Palecek SP. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wn-t/β-catenin signaling under fully defined conditions. Nat Protoc. 2013;8(1): 162–175. doi: 10.1038/nprot.2012.150.
  10. Burridge PW, Matsa E, Shukla P, Lin ZC, Churko JM, Ebert AD, Lan F, Diecke S, Huber B, Mordwinkin NM, Plews JR, Abilez OJ, Cui B, Gold JD, Wu JC. Chemically defined generation of human cardiomyocytes. Nat Methods. 2014;11(8): 855–860. doi: 10.1038/nmeth.2999.
  11. Цвелая ВА. Тканевая инженерия сердца как средство для исследования фундаментальных процессов возникновения реентри [диссертация]. Долгопрудный; 2019. 164 с.
  12. Tsvelaya VA, Gams A, Aziz J, Efimov IR. Induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs): novel diagnostic platform. In: Haider KH, Aziz SM. Stem Cells – from Hype to Real Hope. Berlin: De Gruyter; 2018.
  13. Zhang F, Qu K, Li X, Liu C, Ortiz LS, Wu K, Wang X, Huang N. Gelatin-based hydrogels combined with electrical stimulation to modulate neonatal rat cardiomyocyte beating and promote maturation. Bio-Design and Manufacturing. 2021;4(1): 100–110. doi: 10.1007/s42242-020-00100-9.
  14. Schoen I, Pruitt BL, Vogel V. The Yin-Yang of Rigidity Sensing: How Forces and Mechanical Properties Regulate the Cellular Response to Materials. Annual Review of Materials Research. 2013;43(1): 589–618. doi: 10.1146/annurev-matsci-062910-100407.
  15. Kudryashova N, Nizamieva A, Tsvelaya V, Panfilov AV, Agladze KI. Self-organization of conducting pathways explains electrical wave propagation in cardiac tissues with high fraction of non-conducting cells. PLoS Comput Biol. 2019;15(3):e1006597. doi: 10.1371/journal.pcbi.1006597.
  16. Kadota S, Minami I, Morone N, Heuser JE, Agladze K, Nakatsuji N. Development of a reentrant arrhythmia model in human pluripotent stem cell-derived cardiac cell sheets. Eur Heart J. 2013;34(15): 1147–1156. doi: 10.1093/eurheartj/ehs418.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2021 Shcherbina S.A., Shutko A.V., Nizamieva A.A., Nikitina A.V., Slotvitsky M.M., Tsvelaya V.A., Agladze K.I.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies