The hardware method of biomaterial preparation for fecal transplantation

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Abstract

Background: Nowadays fecal transplantation (FT) is considered as a  component of the treatment for a  wide range of disorders, including autoimmune diseases (ulcerative colitis, Crohn's disease, type 1 diabetes mellitus and insulin resistance, multiple sclerosis, psoriasis). High-quality preparation of the biomaterial is a necessary procedure that allows for long-time storage of the prepared fecal transplant at ultralow temperature conditions and it use as needed.

Aim: To optimize the method of preparation of the biomaterial for fecal transplantation and to evaluate its "survival" at different time points under cryopreservation conditions.

Materials and methods: A device for the preparation of donor fecal material for transplantation has been developed and proposed (the Russian Federation patent No. 2659417 from July 2, 2018). Donor fecal material (collected in a  sterile container on the same day of preparation in the morning), the solvent, and glycerol are homogenized automatically in the closed loop device and passed through a disposable filter with attached sterile hemocon container. Freezing at ultralow temperature (cryopreservation at -80 °C) allows for long time storage of this fecal graft. We studied the microbial composition of the obtained native substrate and samples that were cryopreserved at different time points (7 to 365 days).

Results: The proposed original method makes it possible to prepare the biomaterial for storage at a low temperature mode without any contact, in a  closed loop, for subsequent fecal transplantation within 6–12 months. The analysis of the fecal transplant at different time points has shown no qualitative and quantitative differences in the microbial composition between the native donor material and the freshly prepared filtrate. The biomaterial prepared according to the original method is stable for 12 months.

Conclusion: The proposed hardware method for preparing the biomaterial for fecal transplantation is easy to use and allows for the preparation of a  graft with minimal external microbial contamination, in contrast to the conventional method of donor material preparation by filtering fecal matter through gauze or coffee filters with manual assistance.

About the authors

L. R. Shedoeva

Irkutsk Scientific Center of Surgery and Traumatology

Author for correspondence.
Email: cristal608@yandex.ru
ORCID iD: 0000-0002-6525-3522

Liudmila R. Shedoeva – MD, Coloproctologist, Junior Research Fellow, Laboratory of Reconstructive Surgery, Scientific Department of Clinical Surgery 

1 Bortsov Revolyutsii ul., Irkutsk, 664003

Russian Federation

E. Yu. Chashkova

Irkutsk Scientific Center of Surgery and Traumatology

Email: fake@neicon.ru
ORCID iD: 0000-0002-7953-6523

Elena Yu. Chashkova – MD, PhD, Coloproctologist, Leading Research Fellow, Head of Laboratory of Reconstructive Surgery, Scientific Department of Clinical Surgery 

1 Bortsov Revolyutsii ul., Irkutsk, 664003

Russian Federation

O. G. Karnoukhova

Irkutsk State Medical University

Email: fake@neicon.ru

Olga G. Karnoukhova – MD, PhD, Bacteriologist, Associate Professor, Chair of Pathophysiology and Clinical Laboratory Diagnostics 

1 Krasnogo Vosstaniya ul., Irkutsk, 664003

Russian Federation

G. Yu. Kogan

Irkutsk State Medical University

Email: fake@neicon.ru

Galina Yu. Kogan – MD, Bacteriologist, Assistant, Chair of Pathophysiology and Clinical Laboratory Diagnostics 

1 Krasnogo Vosstaniya ul., Irkutsk, 664003

Russian Federation

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Copyright (c) 2020 Shedoeva L.R., Chashkova E.Y., Karnoukhova O.G., Kogan G.Y.

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